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HIV-1 Genotyping for Resistance Mutation


Test Information

Test Name: HIV-1 Genotyping for Resistance Mutation

Performing Lab: Molecular Diagnostic

Synonyms: Genotype HIV
HIV Genotype

Order Includes: Genotype HIV
HIV Genotype

Turnaround Time: 14 days

Additional Interpretation: Due to its high mutation rate, human immunodeficiency virus (HIV) exists as a complex mixture of genetically related but distinguishable variants. This extreme mutability is attributable to a high replication rate of the virus combined with a high error rate of the viral encoded reverse transcriptase. Wild-type genotypes predominate in the absence of specific selective pressure. Antiretroviral drug therapy usually selects for increasingly resistant HIV variants over the course of treatment. The genotypic interactions are complex, and the database of mutations is evolving as new drug resistance information becomes available.

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Methodology: The ViroSeq HIV-1 Genotyping assay amplifies a 1.8 Kb region of the HIV-1 pol gene that spans the entire protease (PR) gene and approximately two-thirds of the reverse transcriptase (RT) gene. Following the reverse transcription of the target viral RNA, the cDNA is amplified by polymerase chain reaction (PCR). Sequencing of DNA in both directions is performed using dye terminator labeled ddNTPs. Detection of sequencing products is performed in an automated sequencer. Comparison of obtained sequences to wild-type and identification of mutations that confer resistance to antiretroviral drugs is performed.

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CPT Code: 87900
87901

 

 

Result Information

No Information Available

 

 

Specimen & Collection Information

Specimen Name: Plasma

Container Type: BD Vacutainer Pink Top (K2EDTA)

Special Handling: Plasma Stability: REFRIGERATED up to 3 days;FROZEN up to 6 weeks; ROOM TEMP unacceptable.

Patient/Specimen Preparation: Collect whole blood

Specimen Volume: Pin Top: 6 mL of whole blood. Invert 8 to 10 times to mix.

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Specimen Transportation & Storage Information

Storage and Transport:Blood:
Deliver to the lab immediately for processing. Remote Locations : 1) Separate plasma from whole blood within 6 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature. 2)Transfer plasma to a sterile polypropylene tube. 3)Store plasma in the refrigerator at 2-8ºC immediately after separation.
NOTE: before sending the plasma, wrap the tube with an ice pack (previously storage at -20ºC or lower) to ensure the plasma is kept at 2-8ºC during transportation.

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