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HCV RNA Genotyping

Test Information

Test Name: HCV RNA Genotyping

Performing Lab: Molecular Diagnostic

Synonyms: HCV Genotyping

Order Includes: HCV Genotyping

Turnaround Time: 14 days

Additional Interpretation: Hepatitis C is genetically variable, with up to six or more genotypes and several subtypes classified. Some genotypes and subtypes are more likely to respond to interferon treatment than others. To improve response, therapeutic protocols may be varied with respect to pharmaceutical combinations, dosage, and treatment length according to HCV genotype.

Classification of HCV genotype is useful in optimizing therapeutic protocols.

New HCV genotypes/subtypes are still being identified as new information becomes available in the scientific literature.

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Methodology: Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) of target sequences of the 5'UTR and core regions of the HCV genome follow by hybridization of the target sequences to specific capture probes and electromechanical detection with signal probes.

The assay detects eighth of the most prevalent HCV type/subtype: 1a, 1b, 2a/c, 2b, 3, 4 5 and 6

CPT Code: 87902



Result Information

No Information Available



Specimen & Collection Information

Specimen Name: Plasma

Container Type: Lavender Top (K2EDTA)

Special Handling: PLASMA REFRIGERATED up to 3 days;FROZEN at -70ºC or colder up to 6 weeks; ROOM TEMP unacceptable.

Specimen Volume: Lavender top = 3.0 mL

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Specimen Transportation & Storage Information

Storage and Transport:Deliver whole blood to the lab immediately for processing. Plasma MUST be separated from whole blood within 6 hours of collection. Remote Locations : 1)Separate plasma from whole blood within 6 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature.2)Transfer plasma to a sterile polypropylene tube
3)Store plasma in the refrigerator at 2-8ºC immediately after separation.
NOTE: before sending the plasma, wrap the tube with an ice pack (previously storage in the freezer at -20ºC or lower) to ensure plasma is maintain at a temperature between 2-8ºC during transportation.

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