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HIV-1 RNA Quantitative


Test Information

Test Name: HIV-1 RNA Quantitative

Performing Lab: Molecular Diagnostic

Synonyms: HIV 1 Viral Load by PCR
HIV by PCR
HIV RNA PCR
Quant HIV PCR
Viral LoadHIV

Order Includes: HIV 1 Viral Load by PCR
HIV by PCR
HIV RNA PCR
Quant HIV PCR
Viral LoadHIV

Turnaround Time: 7 days

Additional Interpretation: Rapid, direct, and sensitive method for quantifying HIV-1 RNA in plasma to aid in assessing viral response to antiretroviral therapy and indicator of prognosis.

Methodology: Automated RNA extraction from plasma specimens is followed by automated reverse transcription amplification (RT-PCR) and real-time detection of cleaved dual fluorescent dye-labeled oligonucleotide (TaqMan) probes that allow the specific and simultaneous detection and quantification of the target sequence as well as an internal HIV-1 quantification standard with the Roche COBAS TaqMan 96 instrument.

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CPT Code: 87536

 

 

Result Information

No Information Available

 

 

Specimen & Collection Information

Specimen Name: Plasma

Container Type: Pink Top (EDTA)

Special Handling: Plasma stability: REFRIGERATED (2ºC to 8ºC) for up to 6 days; FROZEN (-70ºC) for up to 6 weeks.

Specimen Volume: Pink Top Tube : 6 mL

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Specimen Transportation & Storage Information

Storage and Transport:Deliver whole blood to the Pathology specimen receiving laboratory immediately for processing. Whole blood can be stored refrigerated (2ºC to 25ºC) for no longer than 24 hours. Separate plasma from whole blood within 24 hours of collection by centrifugation at 800-1600xg for 20 minutes at room temperature. Transfer plasma to the molecular polypropylene collection tube. Plasma specimens may be stored at room temperature (25ºC to 30ºC) for up to 1 day, at 2ºC to 8ºC for up to 6 days or frozen at -80ºC for up to 6 weeks. Whole blood must be transported refrigerated and centrifuged within 24 hours of collection.

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