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Oncogenomic Dx One Plus


Test Information

Test Name: Oncogenomic Dx One Plus

Performing Lab: Molecular Diagnostic

Turnaround Time: 14 days

Methodology: DNA isolated from the specimen is amplified for mutational hotspot regions on 50 cancer related genes using next generation sequencing based in AmpliSeq™ technology (Life Technologies, NY).The Oncogenomic Dx One Plus assay detects mutations on selected hotspot regions of the following 50 genes: ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL.

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CPT Code: 81445 (OncoDx1Plus Solid Tumor) or 81450 (OncoDx1Plus Heme)

 

 

Result Information

No Information Available

 

 

Specimen & Collection Information

Specimen Name: Fresh, frozen, or formalin-fixed paraffin-embedded tissue. Whole Blood and bone marrow.

Container Type: Tissue block, Slide holder / sterile container / cryotubes. Lavender Top (EDTA) for whole blood. or bone marrow

Patient/Specimen Preparation: For FFPE: 5-10 unstained slides at 10-micron sections each (air-dried) from the selected paraffin block; and One 5-micron section stained with Hematoxilin and Eosin with tumor area indicated by the pathologist. Whole blood or bone marrow collected in a Lavender EDTA tube

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Specimen Transportation & Storage Information

Storage and Transport:FROZEN TISSUE: Store at -80ºC and transport in dry ice. Do not allow thawing at any time. LASER CAPTURE MICRODISSECTION (LCM): Store and transport in lysis buffer at room temperature within 12 hours of collection. FRESH TISSUE: Store at 4ºC and transport to the lab on a piece of gauze in saline, or in RPMI, within 24 hours of collection. Ship in cooled container during summer months. PARAFFIN-EMBEDDEDD TISSUE: store at room temperature. Transport slides at room temperature within 5-7 days of sectioning. Transport slides in a slide holder. WHOLE BLOOD/BM: Deliver to the lab immediately after collection. DO NOT SPIN.

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