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Engraftment T-Cell Follow-Up


Test Information

Test Name: Engraftment T-Cell Follow-Up

Performing Lab: Molecular Diagnostic

Synonyms: Engr TCell
Engraftment T-Cell Follow Up
T-Cell Engr F-Up

Order Includes: Engr TCell
Engraftment T-Cell Follow Up
T-Cell Engr F-Up

Turnaround Time: 10 days

Additional Interpretation: Chimerism following bone marrow engraftment is a leading cause of morbidity and mortality in the bone marrow transplant population. Peripheral blood cells arising from the recipient as well as the donor appear in the peripheral circulation. As T-cells are the main cell population mediating graft versus host disease, monitoring T-cell chimerism is important in evaluating the immunologic status following a BMT. Therefore, T-cell chimerism studies can be used as a tool in early prediction of graft versus host disease as well as recurrence of the malignant condition.

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Methodology: The T-cell fraction is isolated using Whole Blood CD3+ Microbeads in the Miltenyi Biotec AutoMACS Pro Separator. The extracted DNA from the T-cell fraction is amplified with the 16-locus Human STR panel (Promega PowerPlex 16 HS) and the PCR products are characterized by capillary electrophoresis. The allele profile is then compared to the original profile from donor and recipient for informative alleles. Results for engraftment monitoring of single donor transplant are reported as percentage of donor T-cell cells in the post-transplant sample and calculated from the peak area for each marker in the sample. For a double donor transplant, the identity and the percentage of each donor T-cell cells are reported as well as the total percentage of donor cells.

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CPT Code: 81268

 

 

Result Information

No Information Available

 

 

Specimen & Collection Information

Specimen Name: Whole Blood

Container Type: Pink Top (EDTA)

Special Handling: Refrigerate

Specimen Volume: TWO full pink top collection tubes (6.0 mL of whold blood each).

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Specimen Transportation & Storage Information

Storage and Transport:Blood:
Deliver to the lab immediately. Remote Locations : Blood:
Do not spin. Store refrigerated at 2°C to 8°C.