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Engraftment FollowUp


Test Information

Test Name: Engraftment FollowUp

Performing Lab: Molecular Diagnostic

Synonyms: BM Engraft FUp
Engr FUp
Engraftment Follow Up

Order Includes: BM Engraft FUp
Engr FUp
Engraftment Follow Up

Turnaround Time: 10 days

Additional Interpretation: Chimerism following bone marrow engraftment is a leading cause of morbidity and mortality in the bone marrow transplant population. Peripheral blood cells arising from the recipient as well as the donor appear in the peripheral circulation. Early monitoring of this state is necessary to allow early intervention.

Methodology: Peripheral blood or bone marrow DNA from the recipient in a marrow transplant are amplified with the 16-locus Human STR panel (Promega PowerPlex 16 HS) and the PCR products are characterized by capillary electrophoresis. The allele profile is then compared to the original profile from donor and recipient for informative alleles. Results for engraftment monitoring of single donor transplant are reported as percentage of donor cells in the post-transplant sample and calculated from the peak area for each marker in the sample. For a double donor transplant, the identity and the percentage of each donor cells are reported as well as the total percentage of donor cells.

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CPT Code: 81267

 

 

Result Information

No Information Available

 

 

Specimen & Collection Information

Specimen Name: Whole Blood
Bone Marrow

Container Type: Pink Top (EDTA)
Lavender Top (EDTA)

Special Handling: Refrigerate

Specimen Volume: 4.00 mL of whole blood (pink top collection tube) and/or 0.5 mL of bone marrow (lavender top)

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Specimen Transportation & Storage Information

Storage and Transport:Blood and Bone Marrow:
Deliver to the lab immediately. Remote Locations : Blood and Bone Marrow
Do not spin. Store refrigerated at 2°C to 8°C.